Nian Wang

Associate ProfessorWang

Citrus research and Education Center, Lake Alfred, FL
Department of Microbiology and Cell Science University of Florida

Post-Doctoral (2005-2006): University of California, Berkeley
Ph.D. (2005): Texas A&M University Plant Pathology
M.S. (2001): China Agricultural University, Beijing China Plant Pathology

Contact Information

863 956-1151 ext. 1430
nianwang@ufl.edu

Description of Research

Molecular genetics and functional genomics of plant-bacteria interactions and methods to control citrus diseases caused by Candidatus Liberibacter asiaticus (Citrus Greening) and Xanthomonas axonopodis pv. citri (Citrus Canker)

Selected Publications

PUBLICATIONS (Underline denotes corresponding author).
RESEARCH PAPERS (Refereed) 

2013

  1. Maxuel O. Andrade, Chuck S. Farah, Nian Wang. The post-transcriptional regulator rsmA/csrA activates T3SS by stabilizing the 5′ UTR of hrpG, the master regulator of hrp/hrc genes, in Xanthomonas. PLoS Pathogens. (Accepted).
  2. Trivedi P., Wang N. 2013 Host immune responses accelerate pathogen evolution ISME J. (advance online publication, December 5 2013; doi:10.1038/ismej.2013.215)
  3. Yang Hu, Junli Zhang, Hongge Jia, Davide Sosso, Ting Li, Wolf B. Frommer, Bing Yang, Frank F. White, Nian Wang, and Jeffrey B. Jones Lateral organ boundaries 1 is a disease susceptibility gene for citrus bacterial canker disease Proc Natl Acad Sci U S A. (doi/10.1073/pnas.1313271111).
  4. Wang, N.; Trivedi, P. 2013 Citrus Huanglongbing – an old problem with an unprecedented challenge. Phytopathology. 103: 652-665 (Invited review).
  5. Aritua V, Achor D, Gmitter FG, Albrigo G, Wang N. 2013 Transcriptional and Microscopic Analyses of Citrus Stem and Root Responses to Candidatus Liberibacter asiaticus Infection. PLoS One. 8(9):e73742. doi: 10.1371/journal.pone.0073742.
  6. Jalan N, Kumar D, Andrade MO, Yu F, Jones JB, Graham JH, White FF, Setubal JC, Wang N. 2013 Comparative genomic and transcriptome analyses of pathotypes of Xanthomonas citri subsp. citri provide insights into mechanisms of bacterial virulence and host range. BMC Genomics. doi: 10.1186/1471-2164-14-551.
  7. Li J, Wang N. 2013 Foliar application of biofilm formation inhibiting compounds enhances control of citrus canker caused by Xanthomonas citri subsp. citri. Phytopathology (Epub ahead of print).
  8. Qing Yan, Aswathy Sreedharan, Shiping Wei, Jihua Wang, Kirsten Pelz-Stelinski, Svetlana Folimonova,  Nian Wang 2013 Global gene expression changes in Candidatus Liberibacter asiaticus during the transmission in distinct hosts between plant and insect. Molecular Plant Pathology. 14:391-404.
  9. Jalan N, Kumar D, Yu F, Jones JB, Graham JH, Wang N. Complete Genome Sequence of Xanthomonas citri subsp. citri Strain Aw12879, a Restricted-Host-Range Citrus Canker-Causing Bacterium. Genome Announc. 1(3). doi:pii: e00235-13.
  10. Xiuhua Chen, Jinyoung Y. Barnaby, Aswathy Sreedharan, Xiaoen Huang, Vladimir Orbović, Jude W. Grosser, Nian Wang, Xinnian Dong, Wen-Yuan Song 2013 Over-expression of the citrus gene CtNH1 confers resistance to bacterial canker disease. Physiological and Molecular Plant Pathology 84: 115–122.

2012

  1. Trivedi P., He Z., Van Nostrand J. D., Zhou J., Albrigo L. G., and Wang N. 2012 Huanglongbing alters the structure and functional diversity of microbial communities associated with citrus rhizosphere.  ISME J. 6, 363–383.
  2. Kogenaru, S.,Yan, Q., Guo, Y., and Wang, N. 2012 RNA-seq and microarray complement each other in transcriptome profiling. BMC Genomics 13:629.
  3. Wang N, Li JL, Lindow SE. 2012 RpfF-dependent regulon of Xylella fastidiosa. Phytopathology. 102:1045-53.
  4. Guo, Y., Zhang, Y., Li, J.L., Wang, N. 2012 DSF-mediated quorum sensing plays a central role in coordinating gene expression of Xanthomonas citri subsp. citri. Molecular Plant-Microbe Interactions 25: 165–179.
  5. Yan, Q., and Wang, N. 2012 High-throughput screening and analysis of genes of Xanthomonas citri subsp. citri involved in citrus canker symptom development  Molecular Plant-Microbe Interactions 25:69-84.
  6. Qing Yan, Xiufang Hu, Nian Wang 2012 The novel virulence related gene nlxA is involved in the production of lipopolysaccharide and extracellular polysaccharide, motility, biofilm formation and stress resistance of Xanthomonas citri subsp. citri Molecular Plant Pathology 13:923-34.
  7. Li J., and Wang N. 2012 The gpsX gene encoding glycosyltransferase is important for polysaccharide production and required for full virulence in Xanthomonas citri subsp. citri  BMC Microbiology 12: 31.
  8. Akula, N., Trivedi, P., Han, F.Q., Wang, N. 2012 Identification of small molecule inhibitors against SecA of Candidatus Liberibacter asiaticus by structure based design
    European Journal of Medicinal Chemistry 54:919-24.

2011        

  1. Neha Jalan, Valente Aritua, Dibyendu Kumar, Fahong Yu, Jeffrey B. Jones, James H. Graham, João C. Setubal, and Nian Wang 2011 Comparative genomic analysis of Xanthomonas axonopodis pv. citrumelo F1 causing citrus bacterial spot and related strains provides insights into virulence and host-specificity. Journal of Bacteriology 193:6342-57.
  2. Li, J. and Wang, N. 2011b Genome-wide mutagenesis of Xanthomonas axonopodis pv. citri reveals novel genetic determinants and regulation mechanisms of biofilm formation.  PLoS ONE 6(7): e21804.
  3. Guo, Y., Figueiredo, F., Jones, J., and Wang, N. 2011 HrpG, together with HrpX, play global roles in coordinating different virulence traits of Xanthomonas axonopodis pv. citri  Molecular Plant-Microbe Interactions 12:381-96.
  4. Trivedi, P. Spann, T. and Wang N. 2011 Isolation and Characterization of Beneficial Bacterial Community Associated with Citrus Roots in Florida. Microbial Ecology 62: 324-36.
  5. Akula N, Zheng H, Han FQ, Wang N. 2011 Discovery of novel SecA inhibitors of Candidatus Liberibacter asiaticus by structure based design. Bioorg Med Chem Lett. 21:4183-8.
  6. Yan Q., and Wang, N. 2011a The ColR/ColS two-component system plays multiple roles in the pathogenicity of the citrus canker pathogen Xanthomonas citri subsp. citri Journal of Bacteriology 193:1590-1599.
  7. Li, J. and Wang, N. 2011 The wxacO gene of Xanthomonas citri ssp. citri encodes a protein with a role in lipopolysaccharide biosynthesis, biofilm formation, stress tolerance and virulence  Molecular Plant Pathology 12:381-396.

2010

  1. Guo, Y., Sagaram, U.S., Kim, J.S., and Wang, N2010.  Requirement of the galU gene for polysaccharide production by and pathogenicity and growth in planta of Xanthomonas citri subsp. citriApplied and Environmental Microbiology 76:2234‑2242.
  2. Trivedi, P., Duan, Y., and Wang, N2010.  Huanglongbing, a systemic disease, restructures the bacterial community associated with citrus roots.  Applied and Environmental Microbiology 76: 3427–3436.

2009

  1. Sagaram, U.S., DeAngelis, K.M., Trivedi, P., Andersen, G.L., Lu, S., and Wang, N.  2009.  Bacterial diversity analysis of Huanglongbing pathogen-infected citrus using PhyloChips and 16S rDNA clone library sequencing.  Applied and Environmental Microbiology 75:1566-1574.
  2. Kim, J., Sagaram, U.S., Burns, J.K., and Wang, N.  2009.  Response of sweet orange (Citrus sinensis) to Candidatus Liberibacter asiaticus infection:  microscopy and microarray analyses.  Phytopathology 99:50-57.
  3. Trivedi, P., Sagaram, U.S., Kim, J.S., Brlansky, R.H., Rogers, M., Stelinski, L.L., Oswalt, C., Kim, J.S., and Wang, N.  2009.  Quantification of viable Candidatus Liberibacter asiaticus in hosts using quantitative PCR with the aid of ethidium monoazide (EMA).  European Journal of Plant Pathology 124:553-563.
  4. Gu, G., Smith, L., Wang, N., Wang, H., and Lu, S.  2009a.  Biosynthesis of an antifungal oligopeptide in Burkholderia contaminans strain MS14.  Biochemical and Biophysical Research Communications 380:328-332.
  5. Gu, G., Wang, N., Chaney, N., Smith, L., and Lu, S.  2009b.  AmbR1 is a key transcriptional regulator for production of antifungal activity of Burkholderia contaminans strain MS14.  FEMS Microbiological Letters 297:54-60.
  6. Boina, D., Rogers, M.E., Wang, N., and Stelinski, L.L.  2009.  Effect of pyriproxyfen, a juvenile hormone mimic, on egg hatch, nymph development, adult emergence and reproduction of the Asian citrus psyllid, Diaphorina citri Kuwayama.  Pest Management Science 66: 349-357.

2008

  1. Tatineni, S., Sagaram, U.S., Gowda, S., Robertson, C.J., Dawson, W.O., Iwanami, T., and Wang, N.  2008.  In planta distribution of ‘Candidatus Liberibacter asiaticus’ as revealed by polymerase chain reaction (PCR) and real-time PCR.  Phytopathology 98(5):592‑599.

2006

  1. Wang, N., Lu, S.E., Records, A.R., and Gross, D.C.  2006.  Characterization of the regulatory roles of syrF and salA in syringomycin and syringopeptin production by P. syringae pv. syringae.  Journal of Bacteriology 188:3290-3298.
  2. Wang, N., Lu, S.-E., Wang, J.L., Chen, J.Z., and Gross, D.C.  2006.  The expression of genes encoding lipodepsipeptide phytotoxins by Pseudomonas syringae pv. syringae is coordinated in response to plant signal molecules.  Molecular Plant-Microbe Interactions 19:257‑269.
  3. Wang, N., Lu, S.-E., Yang, Q., Sze, S.-H., and Gross, D.C.  2006.  Identification of the syr-syp box in the promoter regions of genes dedicated to syringomycin and syringopeptin production by Pseudomonas syringae pv. syringae B301D.  Journal of Bacteriology 188:160-168.

2005

  1. Lu, S.E., Wang, N., Wang, J.L., Chen, J.Z., and Gross, D.C.  2005.  Oligonucleotide microarray analysis of the SalA regulon controlling phytotoxin production by Pseudomonas syringae pv. syringae.  Molecular Plant-Microbe Interactions 15:43-53.

 

 

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