Mariola Edelmann Current Projects Fall 2015
Identifying active deubiquitinases and kinases in chicken
Our long-term goal is to provide tools and resources for the identification of regulatory networks in chicken relevant to growth or disease. The specific objective of this proposal is to annotate chicken deubiquitinating enzymes (DUBs) and kinases at a genome-scale by using chemical proteomics. Our overall hypothesis is that chicken genome encodes for multiple DUBs and kinases, which have unique distribution in tissues. The rationale for our study is that the chemical probes that react with catalytically active enzymes can be utilized to determine tissue-specific expression and activity of DUBs and kinases, improving the existing annotation of chicken genome for future research. Experimental annotation of DUBs and kinases, based on their activity will be complementary to the existing computational predictions, which are likely to be incomplete or incorrect.
Host-pathogen interactions in Salmonella andYersinia infection
There is a fundamental knowledge gap in understanding the essential protein-protein interactions in salmonellosis and yersiniosis – which are some of the leading bacterial foodborne diseases. While they can be treated with antibiotics, the high incidence of the multidrug-resistant strains means that development of new treatments is essential. Our long-term goal is to understand the relevance of ubiquitin proteasome system in Salmonella and Yersinia infections to identify novel targets for therapeutic interventions. The overall objective is to identify the protein substrates and elucidate mechanisms of bacterial and human deubiquiitnating enzymes (DUBs) in these infections. The central hypothesis is that human and bacterial DUBs determine the disease outcome via deubiquitination of proteins in specific signaling pathways, such as inflammation. The rationale for this research is that the identification of DUB functions in infection can provide a better understanding of mechanisms of these foodborne diseases.
- How to Apply:
- Send an email to: Dr. Mariola J. Edelmann;email@example.com
Depending on interest, a student can do literature search, bioinformatics analysis or laboratory work (protein extraction, proteomics). A person who can design a website would also be beneficial.