Maupin Research Lab

Description of Research

General area: regulation of cells by proteolytic enzymes and a specialization in proteasomes (multicatalytic nucleotide-dependent proteases) from the methanogenic and halophilic archaea.

Interests also include metabolic engineering of bacteria with emphasis on the structure-function of pyruvate decarboxylase for optimization of its activity in organisms growing at high temperature and low pH.  Her research employs biochemical, molecular, genomic, and structural biology techniques to investigate fundamental aspects of microbial metabolism and physiology.

 

Extreme condition biocatalyst: solvent/thermal tolerant inorganic pyrophosphatase

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Selected Publications

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H.V. Miranda, N. Nembhard, D. Su, N. Hepowit, D.J. Krause, J.R. Pritz, C. Phillips, D. Söll and J.A. Maupin-Furlow. 2011. E1- and ubiquitin-like proteins provide a direct link between protein conjugation and sulfur transfer in archaea. Proc. Natl. Acad. Sci. U.S.A. (published Early Edition – February 28, 2011)

Rawls, K.S., S.K. Yacovone, and J.A. Maupin-Furlow. 2010. GlpR represses fructose and glucose metabolic enzymes at the level of transcription in the haloarchaeon Haloferax volcanii. J. Bacteriol. 192: 6251-6260.

Eichler, J., J. Maupin-Furlow, and J. Soppa. 2010. Archaeal Protein Biogenesis: Posttranslational Modification and Degradation. Archaea 2010:643046.

Humbard, M.A., C.J. Reuter, K. Zuobi-Hasona, G. Zhou, and J.A. Maupin-Furlow. 2010. Phosphorylation and methylation of proteasomal proteins of the haloarcheon Haloferax volcanii. Archaea 2010:481725.

Hartman AL, Norais C, Badger JH, Delmas S, Haldenby S, Madupu R, Robinson J, Khouri H, Ren Q, Lowe TM, Maupin-Furlow J, Pohlschroder M, Daniels C, Pfeiffer F, Allers T, Eisen JA. The complete genome sequence of Haloferax volcanii DS2, a model archaeon. PLoS One. 2010 Mar 19;5(3):e9605

Uthandi, S., B. Saad, M.A. Humbard and J.A. Maupin-Furlow. 2010. LccA, an archaeal laccase secreted as a highly-stable glycoprotein into the extracellular medium of Haloferax volcanii. Appl. Environ. Microbiol. 76:733-743.

Humbard, M. A., H.V. Miranda, J.-M. Lim, D. J. Krause, J. R. Pritz, G. Zhou, S. Chen, L. Wells and J. A. Maupin-Furlow. 2010. Ubiquitin-like small archaeal modifier proteins (SAMPs) in Haloferax volcanii. Nature 463: 54-60.

Uthandi, S., B. Saad, M. A. Humbard and J. A. Maupin-Furlow. 2010. LccA, an archaeal laccase secreted as a highly-stable glycoprotein into the extracellular medium of Haloferax volcanii. Appl. Environ. Microbiol. 76:733-743.

Reuter, C. J., S. Uthandi, J. A. Puentes and J. A. Maupin-Furlow. 2010. Hydrophobic carboxy-terminal residues dramatically reduce protein levels in the haloarchaeon Haloferax volcanii. Microbiology 156:248-55.

Humbard, M. A., G. Zhou and J. A. Maupin-Furlow. 2009. N-terminal penultimate residue of 20S proteasome α1 influences its Nα-acetylation and protein quantity as well as the thermotolerance and hypoosmotic stress response of Haloferax volcanii. J. Bacteriol. 191:3794-803.

Sherwood, K. E., D. J. Cano and J. A. Maupin-Furlow. 2009. Glycerol-mediated repression of glucose metabolism and glycerol kinase as the sole route of glycerol catabolism in the haloarchaeon Haloferax volcaniiJ. Bacteriol. 191:4307-15.

Kirkland, P. A. and J. A. Maupin-Furlow. 2009. Stabilization of an archaeal DNA sliding clamp protein, PCNA, by proteasome-activating nucleotidase gene knockout in Haloferax volcanii FEMS Microbiol. Lett. 294:32-6.

Zhou, G., D. Kowalczyk, M. A. Humbard, S. Sohatgi and J. A. Maupin-Furlow. 2008.  Proteasomal components required for cell growth and stress responses in the haloarchaeon Haloferax volcaniiJ. Bacteriol. 190:8096-8105.

Kirkland, P. A., M. A. Humbard, C. J. Daniels and J. A. Maupin-Furlow. 2008.  Shotgun proteomics of the haloarchaeon Haloferax volcaniiJ. Proteome Res. 7:5033-5039.

De Castro, R. E., D. M. Ruiz, M. I. Giménez, M. X. Silveyra, R. A. Paggi and J.A. Maupin-Furlow. 2008.  Gene cloning and heterologous synthesis of a haloalkaliphilic extracellular protease of Natrialba magadii (Nep).  Extremophiles 12:677-687.

Bidle, K. A., P. A. Kirkland, J. Nannen and J. Maupin-Furlow. 2008. Proteomic analysis of Haloferax volcanii reveals up-regulation of the transcriptional activator PspA in response to salt stress.  Microbiology 154:1436-1443.

Kirkland, P. A., M. A. Gil, I. M. Karadzic and J. A. Maupin-Furlow. 2008. Genetic and proteomic analyses of a proteasome-activating nucleotidase A mutant of the haloarchaeon Haloferax volcaniiJ. Bacteriol. 190:193-205.

Gil, M. A., K. E. Sherwood, and J. A. Maupin-Furlow. 2007. Transcriptional linkage of Haloferax volcanii proteasomal genes with non-proteasomal gene neighbors including RNase P, MOSC domain and SAM-methyltransferase homologs. Microbiology (in press).

Kirkland, P. A., C. J. Reuter, and J. A. Maupin-Furlow. 2007. Effect of proteasome inhibitor clasto-lactacystin- -lactone on the proteome of the haloarchaeon Haloferax volcaniiMicrobiology 153: 2271-2280.

Maupin-Furlow, J. A., M. A. Humbard, P. A. Kirkland, W. Li, C. J. Reuter, A. J. Wright, and G. Zhou. 2006.  Proteasomes from structure to function: perspectives from archaea.  G. Schatten (ed.). 2006.  Curr. Top. Dev. Biol. 75: 125-169 (front cover photo) [medline]

Humbard, M. A., S. Stevens Jr., and J. A. Maupin-Furlow. 2006.  Post-translational modification of the 20S proteasomal proteins of the archaeon Haloferax volcanii.  J. Bacteriol. 188:7521-30 [medline]

Dinitz-Bab, E., H. Shmuely, J. Maupin-Furlow, J. Eichler and B. Shaanan. 2006.  Haloferax volcanii PitA:  An example of functional interaction between the Pfam chlorite dismutase and antibiotic biosynthesis monooxygenase families?  Bioinformatics 22:671-675 [medline].

Kirkla, P. A., J. Busby, S. Stevens, and J. A. Maupin-Furlow.nd 2006.  Advances in isoelectric focusing of halophilic proteins.  Anal. Biochem. 351: 254–259 [medline].

Maupin-Furlow, J. A., M. A. Gil, M. A. Humbard, P. A. Kirkland, W. Li, C. J. Reuter, and A. J. Wright. 2006.  Proteasomes and other nanocompartmentalized proteases in archaea.  J. M. Shively (ed.) Springer-Verlag.  Microbiology Monographs:  Complex Intracellular Structures in Prokaryotes, pp. 23 – 46 [weblink].

De Castro, R. E., J. A. Maupin-Furlow, M. I. Giménez, M. K. Herrera Seitz, and J. J. Sánchez. 2006. Haloarchaeal proteases and proteolytic systems. FEMS Microbiol. Rev. 30:17-35. [medline]

Talarico, L. A., M. A. Gil, L. P. Yomano, L. O. Ingram, and J. A. Maupin-Furlow. 2005. Construction and expression of ethanol production operon in gram-positive bacteria. Microbiology 151: 4023-4031. [medline].

Maupin-Furlow, J. A., M. A. Gil, P. A. Kirkland, M. A. Humbard, W. Li, C. J. Reuter, and A. J. Wright. 2005.  Archaeal proteasomes and other regulatory proteases.  Curr. Opin. Microbiol. 8:720-728. [medlin].

Karadzic, I. M. and J. A. Maupin-Furlow. 2005.  Improvement of two-dimensional gel electrophoresis proteome maps of the haloarchaeon Haloferax volcanii. Proteomics 5:354-359. [medline].

Kaczowka, S. J., C. J. Reuter, L. A. Blalock and J. A. Maupin-Furlow.  2005.  Recombinant Production of Zymomonas mobilis Pyruvate Decarboxylase in the haloarchaeon Haloferax volcanii. Archaea 1:327-34 [medline].

Reuter, C. J. and J. A. Maupin-Furlow.  2004.  Analysis of proteasome-dependent proteolysis in Haloferax volcanii cells using short-lived green fluorescent proteins. Appl. Envir. Microbiol. 70:7530-7538.[medline]

Reuter, C. J., S. J. Kaczowka and J. A. Maupin-Furlow.  2004.  Differential regulation of PanA and PanB proteasome-activating nucleotidase and 20S proteasome proteins of the haloarchaeon Haloferax volcanii. J. Bacteriol.186:7763-7772. [medline]

Maupin-Furlow, J. A., M. A. Gil, I. M. Karadzic, P. A. Kirkland, and C. J. Reuter.  2004. Proteasomes:  perspective from the Archaea [update 2004].  Front Biosci. 9:1743-1758. [medline]

Maupin-Furlow, J. A., S. J. Kaczowka, C. J. Reuter, K. Zuobi-Hasona, and M. A. Gil. 2003. Archaeal proteasomes:  potential in metabolic engineering.  Metab. Eng. 5:151-163. [medline]

Kaczowka, S. J. and J. A. Maupin-Furlow. 2003.  Subunit topology of two 20S proteasomes from Haloferax volcanii.  J. Bacteriol. 185:165-174. [medline].

Raj, K.C., Talarico, L. A., Ingram, L. O., and J. A. Maupin-Furlow.  2002.  Cloning and characterization of the Zymobacter palmae pyruvate decarboxylase (pdc):  comparison to bacterial homologues. Appl. Environ. Microbiol. 68:2869-2876. [medline].

Raj, K. C., Ingram, L.O., and J. A. Maupin-Furlow. 2001. Pyruvate decarboxylase: a key enzyme for the oxidative metabolism of lactic acid by Acetobacter pasteurianus. Arch. Microbiol. 176:443-451. [medline]

Talarico, L., Ingram, L.O., and J. A. Maupin-Furlow. 2001. Production of the gram-positive Sarcina ventriculi pyruvate decarboxylase in Escherichia coli. Microbiology 147:2425-2435. [medline]

Maupin-Furlow, J. A., Kaczowka, S. J., Ou, M. S., and H. L. Wilson. 2001. Archaeal proteasomes: nanocompartments of the cell. Adv. Appl. Microbiol. 50:279-338. [medline]

Maupin-Furlow, J. A., H. L. Wilson, S. J. Kaczowka, and M. S. Ou. 2000. Archaeal proteasomes: from structure to function. Front. Biosci. 5:D837-865. [free full text] [medline]

Wilson, H. L., M. S. Ou, H. A. Aldrich, and J. A. Maupin-Furlow. 2000. Biochemical and physical properties of the Methanococcus jannaschii 20S proteasome and PAN, a homolog of the ATPase (Rpt) subunits of the eucaryal 26S proteasome. J. Bacteriol. 182: 1680-1692. [medline]

Wilson, H. L., H. A. Aldrich, and J. A. Maupin-Furlow. 1999. Halophilic 20S proteasomes of the archeon Haloferax volcanii: purification, characterization, and gene sequence analysis. J. Bacteriol. 181: 5814-5824. [medline]

Maupin-Furlow, J. A., H. C. Aldrich, and J. G. Ferry. 1998. Biochemical characterization of the 20S proteasome from the methanoarchaeon Methanosarcina thermophila. J. Bacteriol. 180:1480-1487. [medline]

Maupin-Furlow, J. A., and Ferry, J. G. 1996. Analysis of the CO dehydrogenase/acetyl-coenzyme A synthase operon of Methanosarcina thermophila. J. Bacteriol. 178:6849-6856. [medline]

Maupin-Furlow, J. A. and Ferry, J. G. 1996. Characterization of the cdhD and cdhE genes encoding subunits of the corrinoid/iron-sulfur enzyme of the CO dehydrogenase complex from Methanosarcina thermophila. J. Bacteriol. 178:340-346. [medline]

Maupin-Furlow, J. A. and Ferry, J. G. 1995. A proteasome from the methanogenic archaeon Methanosarcina thermophila. J. Biol. Chem. 270:28617-28622. [medline]

Ferry, J. G., and J. A. Maupin-Furlow. 1995. Analysis of genes in the pathway for the fermentation of acetate to methane by Methanosarcina thermophila. In Microbial growth on C1 compounds. M. Lidstrom, and R. Tabita (ed.), Kluwer Academic, Dordrecht. p. 64-71.

Maupin-Furlow, J. A., J. K. Rosentel, J. H. Lee, U. Deppenmeier, R. P. Gunsalus, and K. T. Shanmugam. 1995. Genetic analysis of the modABCD (molybdate transport) operon of Escherichia coli. J. Bacteriol. 177:4851-4856. [medline]

Rosentel, J. K., F. Healy, J. A. Maupin-Furlow, J. H. Lee, and K. T. Shanmugam. 1995. Molybdate and regulation of mod (molybdate transport), fdhF and hyc (formate hydrogenlyase) operons in Escherichia coli. J. Bacteriol. 177:4857-4864. [medline]

Maupin, J. A., and K. T. Shanmugam. 1990. Genetic regulation of formate hydrogenlyase of Escherichia coli: role of the fhlA gene product as a transcriptional activator for a new regulator gene, fhlB. J. Bacteriol. 172:4798-4806. [medline]

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